Indirect immunostaining of PFA fixed HEK cells expressing EGFP fusion constructs of the C-terminal end of human CD45 (left column) or mouse CD45 (right column) with top row: rabbit anti-human CD45 (cat. no. HS-427 003; 1 : 500; red) or bottom row: rat anti-mouse CD45 (cat. no. HS-427 017, dilution 1 : 500; red). Rabbit anti-human CD45 is specific for the human protein and does not bind to mouse CD45. Nuclei have been visualized by DAPI staining (blue).

Immunohistochemical staining of formalin fixed paraffin embedded sections of
A: human spleen, B: mouse spleen and
C: rat spleen showing species specificity of rabbit anti-CD45 (cat. no. HS-427 003; 1 : 1000, DAB) for human nucleated hematopoietic cells. Rabbit anti-CD45 is not cross-reacting with murine and rat cells and is suited to distinguish human from murine leukocytes in humanized mouse models. Nuclei have been visualized by haematoxylin staining (blue).

Immunohistochemical staining of formalin fixed paraffin embedded sections of A: mouse liver engrafted with human cells or B: wild type mouse liver with rabbit anti-CD45 (cat. no. HS-427 003; 1 : 1000, DAB). Rabbit anti-CD45 is suited to detect human leukocytes in humanized mouse tissues. Nuclei have been visualized by haematoxylin staining (blue).

Immunohistochemical staining of formalin fixed paraffin embedded sections of A: mouse lung engrafted with human cells or B: wild type mouse lung with rabbit anti-CD45 (cat. no. HS-427 003; 1 : 1000, DAB). Rabbit anti-CD45 is suited to detect human leukocytes in humanized mouse tissues. Nuclei have been visualized by haematoxylin staining (blue).